Transfection is the temporary introduction of foreign nucleic acids into cells, where the gene is expressed from an episomal plasmid for a short period (typically 1–7 days) without integrating into the host genome.
Lentiviral transduction
Lentiviral transduction is the process of using a disabled lentivirus to deliver and stably integrate a gene of interest into a target cell’s genome for long-term expression
qPCR / RT-qPCR
A laboratory protocol for quantitative gene expression analysis, including data normalization and result interpretation
Cell Culture
A practical guide to mammalian cell culture, covering cell maintenance, aseptic techniques, and culture monitoring
MTT Assay
A protocol for assessing cell viability and evaluating the cytotoxic effects of compounds using the MTT assay
CRISPR/Cas9
A step-by-step guide to CRISPR/Cas9-based genome editing for targeted genetic modifications
siRNA Transfection
A practical protocol for introducing siRNA into cells to investigate gene function and regulate gene expression
MTT Assay
A protocol for assessing cell viability and evaluating the cytotoxic effects of compounds using the MTT assay
